Subgingival depuraion, vill först at den behandla munhygienen men får ha i Removal of calculus and bacterial plaque will eliminate or markedly reduce the 

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Samples of subgingival plaque and gingival crevicular fluid were also taken. Plaque samples were analyzed with a DNA-DNA hybridization technique.

78. Kageyama S  Materials and Methods Gingival tissue and subgingival plaque samples were collected from 21 periodontitis patients including 48 periodontal pocket sites with  av N Schwech — Plaque samples were taken before pellicle, dental plaque, fluorometer, protease, gingivalvätska ger mineraler för mineralisering av subgingival tandsten. 57946T · Prevotella fusca · Human oral cavity, periodontic subgingival plaque · J.Downes, Kings College, London, UK · 2009-04-06  51937T · Prevotella multiformis · Human subgingival plaque · T.Kudo, JCM, Saitama, Japan · 2005-10-31 · 51939 · Streptomyces · Industry, environment  Samples of subgingival plaque and gingival crevicular fluid were also taken. Plaque samples were analyzed with a DNA-DNA hybridization technique. T. forsythia, P. nigrescens and C. rectus in the sub-gingival and atherosclerotic plaque samples suggesting that the micoorganisms in the subgingival plaque  The objective of this study was to determine whether the supragingival plaque samples of severe-ECC (S-ECC) patients harbor more EBV load than the  plaque samples suggesting that the micoorganisms in the subgingival plaque had significant chance of their presence in atherosclerotic plaque as well  Microbial analysis of subgingival plaque samples compared to that of Haririan, Hady; Andrukhov, Oleh; Bertl, Kristina; Lettner, Stefan; Kierstein, Sonja; Moritz,  Sugar-free chewing gum helps neutralise plaque acids.

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Differences between pos-itive and negative control were not significant (P <0.05). Conclusion: The novel low abrasive air polishing powder is 2017-08-11 · Objectives The purpose of this study was to compare microbial profiles of saliva, pooled and site-specific subgingival samples in patients with periodontitis. We tested the hypotheses that saliva can be an alternative to pooled subgingival samples, when screening for presence of periopathogens. Design Site specific subgingival plaque samples (n = 54), pooled subgingival plaque samples (n = 18 For each subject, one pooled subgingival sample from the deepest pocket in each quadrant (PD ≥ 5 mm) and another from 4 healthy sites (PD ≤ 3 mm; no attachment loss) were obtained, using sterile paper points.

Two Subgingival Plaque-Sampling Strategies Used With RNA Probes. Autores: Diana-Maria Krigar Localización: Journal of periodontology, ISSN 0022-3492, Vol. 78, Nº. 1, 2007, págs. 72-78

We'll Refund the Prevotella heparinolytica. Subgingival dental plaque. Prevotella histicola. Human  av Å Sullivan · Citerat av 2 — med subgingival depuration utvärderades nyligen samples from patients with refractory periodontitis.

Subgingival plaque sampling

Methods: In 50 patients with periodontitis, subgingival plaque was sampled from the deepest pocket of each quadrant by using paper points and by gaining saliva with saline mouthrinse. Analysis was performed using a commercially available polymerase chain reaction test for 11 periodontal pathogens.

(4): 399–406. 78. Kageyama S  Materials and Methods Gingival tissue and subgingival plaque samples were collected from 21 periodontitis patients including 48 periodontal pocket sites with  av N Schwech — Plaque samples were taken before pellicle, dental plaque, fluorometer, protease, gingivalvätska ger mineraler för mineralisering av subgingival tandsten.

Subgingival plaque sampling

Background: The purpose of this study was to compare the results of microbiological RNA‐probe analysis after subgingival plaque sampling applying two different strategies. Methods: In 220 patients, 2018-09-19 In 25 subjects, one healthy, gingivitis and periodontitis site was sampled for GCF using a strip followed by subgingival plaque sampling with a curette. Bacterial assays indicated that GCF strips removed significant numbers of bacteria when placed intracrevicularly for 5 s. Two Subgingival Plaque‐Sampling Strategies Used With RNA Probes.
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2015-04-01 · Indeed, due to the close contact of plaque with cells from the periodontal tissues and crevicular fluid, human DNA can be incorporated during subgingival plaque sampling. If “contamination” of the sample is relatively high, human DNA may dominate sequencing efforts resulting in a low coverage of microbial DNA, hampering analysis of the microbiome. Subgingival sampling Supragingival plaque should be removed with cotton pellets or periodontal scalers to secure noncontaminated subgingival specimens.

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Collection of subgingival plaque samples with paper points is the common method for determination of periodontal pathogens. 21,24,25, 27, 28 However, sampling subgingival plaque with paper points

2015-04-01 · Indeed, due to the close contact of plaque with cells from the periodontal tissues and crevicular fluid, human DNA can be incorporated during subgingival plaque sampling. If “contamination” of the sample is relatively high, human DNA may dominate sequencing efforts resulting in a low coverage of microbial DNA, hampering analysis of the microbiome. Subgingival sampling Supragingival plaque should be removed with cotton pellets or periodontal scalers to secure noncontaminated subgingival specimens.


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been used to sample subgingival plaque. The tech-nique used may influence the outcome of the mi-crobiological analysis.5,6 Also, the number of sample sites is essential, especially when samples are pooled.7 Microbial subgingival sampling is time con-suming and involves the selection of sampling sites, isolation and drying of these sites, and

Supragingival plaque was removed prior to sampling using sterile cotton pellets.

Comparison of curet and paper point sampling of subgingival bacteria as analyzed by real-time polymerase chain reaction. J Periodontol 2007 

This study aimed to make a comparison of two sampling strategies of subgingival plaque after combined mechanical-antibiotic periodontal therapy.

No toothpaste was used. Methods: In 50 patients with periodontitis, subgingival plaque was sampled from the deepest pocket of each quadrant by using paper points and by gaining saliva with saline mouthrinse. Analysis was performed using a commercially available polymerase chain reaction test for 11 periodontal pathogens. This study aimed to make a comparison of two sampling strategies of subgingival plaque after combined mechanical-antibiotic periodontal therapy. Thirty patients (18 female) suffering from aggressive (n = 12) or generalised severe chronic (n = 18) periodontitis were included. Aggregatibacter actinomycetemcomitans had been detected subgingivally in all prior to anti-infective therapy (AT) and METHODS: In 100 patients with aggressive/chronic periodontitis, subgingival plaque was sampled from the deepest pockets per quadrant (MT4) and per sextant (MT6).